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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: Arch Biochem Biophys. 2008 Oct 7;480(2):85–94. doi: 10.1016/j.abb.2008.09.016

Fig. 4.

Fig. 4

4-HNE-induced activation of Bax, p21, JNK, and p-JNK in RPE and ARPE-19 cells: The expression of Bax (a) p21 (b) p-JNK (c), and JNK (d) in RPE and ARPE-19 cells exposed to different concentrations of 4-HNE. RPE and ARPE-19 cells were treated with 4-HNE (0-50 lM) for 2 h in serum-free medium. Cell extracts (50 μg protein) were resolved on 4-20% SDS-PAGE and immunoblotted. The blots were probed with anti-Bax, anti-p21, anti-JNK, and anti-p-JNK antibodies, respectively. GAPDH (e) was used as the loading control. Blots were developed with West Pico-chemi luminescence reagent (Pierce).