Effect of RLIP76 transfection on 3H-DNPSG and 3H-GSHNE transport in inside-out vesicles (IOV) prepared from K562 cells The overexpression of RLIP76 in K562 cells was confirmed by Western blot analyses using anti-RLIP76 IgG. β-actin was used as internal control (inset). ATP-dependent uptake of 3H-DNPSG (panel A) and 3H-GSHNE (panel B) were determined in inside-out plasma membrane vesicles prepared from control, vector (pcDNA3)-transfected and RLIP76-transfected K562 cells. Standard conditions were 20 μg IOV protein, 10 min incubation time, 37°C temperature, 40 mM sucrose, 4 mM ATP and 100 μM DNPSG or 10 μM GSHNE. Each value represents mean of triplicate determinations with error bars representing standard deviations.