Table IV.
Fraction | 3H-DNPSG transport | 3H-GSHNE transport | IC50 for 4-HNE |
---|---|---|---|
K562 cells purified RLIP76 | 39.4±2.6 nmol/min/mg | 4.2±0.3 nmol/min/mg | |
Recombinant purified RLIP76 | 125±14 nmol/min/mg | 4.8±0.4 nmol/min/mg | |
IOVs from: | |||
Control K562 cells | 147±11 pmol/min/mg | 51 ±4 pmol/min/mg | 1.8±0.2μM |
Vector-transfected K562 cells | 166±18 pmol/min/mg | 56±6 pmol/min/mg | 2.0±0.2μM |
RLIP76-transfected K562 cells | 738±42 pmol/min/mg | 294±31 pmol/min/mg | 9.4±0.6μM |
Inside-out vesicles were prepared from K562 cells according to the protocol as described by us previously (8). RLIP76 was purified by DNP-SG affinity column chromatography from K562 cells as well as from recombinant source and reconstituted into artificial liposomes to perform transport studies.