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. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: Circ Res. 2008 May 29;103(1):80–88. doi: 10.1161/CIRCRESAHA.108.176057

Phenotyping of human EPCs. A: A single outgrowth colony formed 2–3 weeks after seeding of mononuclear cells on fibronectin-coated plates (4X magnification). B: Around week 4, confluent cells grew into a monolayer with cobblestone appearance (10X magnification). C: FCAS analysis of cell surface markers on EPCs. Shown are representative data from at least 3 independent experiments for each marker. The open black-lined histograms represent test antibodies, and filled histograms represent the control IgG antibodies. Percentage positive cells are shown in each marker panel. D: Cells were cultured in EBM-2 for 24 h, and assayed for total NOS activity. n=4–7, *P<0.05, compared with CAECs. E: Western blotting for eNOS in cells in the presence or absence of TNF-α for 24 h. n=4, * P<0.05.

Phenotyping of human EPCs. A: A single outgrowth colony formed 2–3 weeks after seeding of mononuclear cells on fibronectin-coated plates (4X magnification). B: Around week 4, confluent cells grew into a monolayer with cobblestone appearance (10X magnification). C: FCAS analysis of cell surface markers on EPCs. Shown are representative data from at least 3 independent experiments for each marker. The open black-lined histograms represent test antibodies, and filled histograms represent the control IgG antibodies. Percentage positive cells are shown in each marker panel. D: Cells were cultured in EBM-2 for 24 h, and assayed for total NOS activity. n=4–7, *P<0.05, compared with CAECs. E: Western blotting for eNOS in cells in the presence or absence of TNF-α for 24 h. n=4, * P<0.05.

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