. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: J Liq Chromatogr Relat Technol. 2008 Jan 1;31(9):1346–1357. doi: 10.1080/10826070802019913

Table 1.

Two-phase solvent systems and test samples

Two-phase solvent systems^* (volume ratio)	Settling time (s)	Test samples (weight)	K (UP/LP)^**
1-BuOH/AcOH/H₂O (4:1:5)	25	Trp-Trp (1 mg)	4.55
		Trp-Tyr (1 mg)	1.69
		Leu-Tyr (4 mg)	0.85
		Val-Tyr (4 mg)	0.53
		Tyr-Gly (4 mg)	0.31
Hexane/EtOAc/MeOH/0.1M HCl (1:1:1:1)	19	DNP-L-Ala (0.5 mg)	2.36
		DNP-β-ala (0.5 mg)	1.18
		DNP-L-glu (0.5 mg)	0.44
Hexane/EtOH/H₂O (5:4:1)	9	Sudan II (1 mg)	2.28
		Sudan I (1 mg)	1.95
Hexane/acetonitrile	9	Sudan II (1 mg)	0.77
		Sudan I (1 mg)	0.39

BuOH: buthanol; AcOH: acetic acid; EtOAc: ethyl acetate; MeOH: methanol; EtOH: ethanol; H₂O: water

^**

K: partition coefficient; UP: upper phase; LP: lower phase