Table 2.
Partition efficiency and stationary phase retention of polar two-phase solvent system in spiral HSCCC
Two-phase solvent system: 1-BuOH/AcOH/H20 (4:1:5)
Sample: trp-trp (1 mg), trp-tyr (1 mg), leu-tyr (4 mg), val-tyr (4 mg), tyr-gly (4 mg) in 1 ml solvent (UP 0.4 ml + LP 0.6 ml)
Column: Sprial tube assembly, 1.6 mm ID, cross-pressed, 4 radial grooves compressed, 12 layers, 100 ml capacity, embedded in PEG3350
| Elution mode | Flow rate (ml/min) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 5 | |||||||
| TP | RS | Sf% | TP | RS | Sf% | TP | RS | SF% | |
| LIH | 414/292/195/194/- | 2.93 | 50 | 676/415/198/210/- | 2.48 | 44 | -/-/-/189/145 | - | 29 |
| LIT | 784/442/299/392/- | 4.23 | 52 | 1810/661/360/391/- | 3.61 | 43 | -/-/1079/406/344 | - | 14 |
| UOT | 522/331/252/290/191 | 2.66 | 39 | 345/333/210/256/166 | 2.12 | 30 | -/-/-/-/169 | - | 19 |
| UOH | 1024/784/351/398/623 | 3.98 | 70 | 900/816/492/622/442 | 3.69 | 50 | -/1764/1239/917/375 | 3.48 | 32 |
TP: theoretical plate number (1st/2nd/3rd/4th/5th peaks); Rs: peak resolution between the 2nd and the 4th peaks; Sf: stationary phase retention - : measurement was not made.