Table 3.
Partition efficiency and stationary phase retention of moderately hydrophobic two-phase solvent system in spiral HSCCC
Two-phase solvent system: Hexane/EtOAc/MeOH/0.1M HCl (1:1:1:1)
Sample: DNP-glu, DNP-β-ala, DNP-ala, 0.5 mg each
Column: Spiral tube assembly, 1.6 mm ID, cross-pressed, 4 radial grooves compressed, 12 layers, 100 ml capacity, embedded in PEG3350
| Elution mode | Flow rate (ml/min) | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 5 | |||||||
| TP | Rs | Sf % | TP | Rs | Sf % | TP | Rs | Sf % | |
| LIH | 757/614/423 | 3.18/2.38 | 60 | 816/480/400 | 2.78/2.23 | 57 | 798/621/506 | 2.33/1.91 | 46 |
| LIT | - | - | ≈ 0 | - | - | ≈ 0 | - | - | ≈ 0 |
| LOH | 666/576/391 | 2.47/2.07 | 46 | 667/625/463 | 2.85/2.00 | 45 | 576/529/457 | 1.43/1.63 | 32 |
| UOT | 111/145/154 | 1.36/2.51 | 86 | 108/126/130 | 1.22/2.24 | 74 | 176/135/136 | 1.23/2.14 | 68 |
| UOH | 4225/3136/1965 | 1.11/2.77 | 18 | 9526/6864/3095 | 1.00/2.40 | 13 | 5476/3184/2500 | 1.04/2.42 | 10 |
| UIT | 253/294/324 | 1.93/3.48 | 83 | 204/171/138 | 1.18/2.28 | 76 | 164/247/300 | 1.13/1.24 | 38 |
TP: theoretical plate number (1st peak/2nd peak/3rd peak); Rs: peak resolution (1st & 2nd/2nd & 3rd peaks); Sf: stationary phase retention