. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: J Liq Chromatogr Relat Technol. 2008 Jan 1;31(9):1346–1357. doi: 10.1080/10826070802019913

Table 6.

Choise of the elution mdoe for 4 typical two-phase solvent systems in spiral HSCCC

Two-phase solvent system^* (volume ratio)	settling time (second)	choice of the elution mode^**
Two-phase solvent system^* (volume ratio)	settling time (second)	Lower mobile phase	Upper mobile phase	U vs.L
1-BuOH-AcOH-H₂O (4:1:5)	>30	LIT>>LIH	UOH>>UOT	LIT≈UOH
Hexane-EtOAc-MeOH-0.1M HCl (1:1:1:1)	19	LIH>>LOH	UIT>UOT>UOH	LIH>UIT
Hexane-EtOH-H₂O (5:4:1)	9	LOH≤LIH	UIT>UOT	LOH>>UIT
Hexane-acetonitrile	9	LOH>LIH	UIT>>UOT	LOH<UIT

BuOH: butanol; AcOH: acetic acid; EtOAc: ethyl acetate; MeOH: methanol; EtOH: ethanol

^**

L: lower mobile phase; U: upper mobile phase; I: internal terminal of the spiral channel; O: outer terminal of the spiral channel; H: head of the spiral channel; T: tail of the spiral channel.