Activation of PMNs with fMLP increases caveolae-mediated transendothelial 125I-albumin permeability in RLMVEC monolayers. A, Effect of PMNs in the absence and presence of fMLP on transendothelial 125I-albumin permeability. B, Activation of PMNs with fMLP increased transendothelial 125I-albumin permeability in a PMN number-dependent manner. C, Effect of pretreatment of RLMVECs with MβCD on 125I-albumin permeability. The baseline permeability value for control group (without PMNs and fMLP) is 9.4±1.1 cm/sec. D, Effect of activation of PMNs (107 cells/mL) with fMLP (1.0 μmol/L) or PMA (0.1 μmol/L) on TER. Results are typical of 3 experiments (A through C). n=4 to 6 for each group. *P<0.05 compared with control group (without PMNs and fMLP), †P<0.05 compared with respective groups.