Activation of PMNs with fMLP increases caveolae-mediated endocytosis of albumin in endothelial cells. A, Activation of PMNs with fMLP increased 125I-albumin endocytosis in a PMN number-dependent manner. B, Time course of effects of fMLP activation of PMNs (107 cells/mL) on 125I-albumin endocytosis. C, Effect of pretreatment of RLMVECs with MβCD on PMN-induced (107 cells/mL) 125I-albumin endocytosis. Results are representative of 3 experiments. Scale bars=10 μm. n=4 to 6 for each group (A and C) and time point (B). *P<0.05 compared with control group (without PMNs and fMLP), †P<0.05 compared with respective groups. D, Confocal images showing effect of fMLP activation of PMNs induced a PMN number-dependent increase in uptake of Alexa 488–labeled albumin (green). The nucleus (blue) was stained with DAPI. Results are representative of 3 experiments.