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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Circ Res. 2008 May 29;102(12):e120–e131. doi: 10.1161/CIRCRESAHA.107.167486

Figure 7.

Figure 7

ICAM-1 crosslinking induces Src activation and Cav-1 phosphorylation and activates albumin endocytosis and transcytosis. RLMVECs were incubated with 10 μg/mL anti–ICAM-1 Ab at 37°C for 30 minutes and washed and incubated with 0.1 μg/mL goat antimouse IgG F(ab′)2 at 37°C for 5, 10, and 30 minutes. A and supplemental Figure IIC, Effects of ICAM-1 crosslinking on Src activation and Cav-1 phosphorylation. B, Effects of ICAM-1 crosslinking on Alexa 488 –labeled albumin uptake (green) and anti–ICAM-1 IgG+anti-mouse Alexa 568 immunostaining (red). Confocal images show ICAM-1 clustering and increased tracer albumin uptake following ICAM-1 crosslinking. Scales bar=10 μm. C and D, Time course of ICAM-1 crosslinking effect on 125I-albumin endocytosis (C) and transcytosis (D). Results are typical of 3 experiments (A and B). n=4 to 6 for each time point (C and D). *P<0.05 compared with control group.