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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Endocrinology. 2007 May 17;148(8):3758–3764. doi: 10.1210/en.2006-1650

Figure 3.

Figure 3

Phosphorylation of STAT3 in the anterior pituitary in response to acute stress. In the anterior pituitary there was lack of immunoreactivity in the non-immune control (A) and positive immunoreactivity for POMC (B). The ratio of pSTAT3 to STAT3 was determined by Immunoblot and demonstrated an increase in phosphorylation of STAT3 in response to exhaustive exercise (C; *P < 0.05; n = 4). The increase in STAT3 phosphorylation is also demonstrated by immunohistochemistry showing increased nuclear pSTAT3 after exercise (B, n = 3). Dual immunohistochemical results for pSTAT3 (brown) and POMC (violet) in control and acute stress animals (E). (E) Blue arrows point to POMC positive cells demonstrating nuclear immunoreactivity for pSTAT3. C, control; S, stress.