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Northern analyses of Nek1 expression in control and mutant animals. Lanes 1–3 contain poly(A)+ RNA from the testis, and lanes 4–6 contain poly(A)+ RNA from the kidneys of 3-month-old animals. A was probed with a hybrid-selected Nek1 cDNA, clone26A, that represents 1838–2325 nt of the Nek1 transcript (19). B represents the same blot stripped and probed with a 1.8-kb human β-actin cDNA fragment, a sample loading control. Size markers used to estimate kilobases were 28S and 18S RNA.
