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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Cancer Res. 2009 Jan 1;69(1):37–44. doi: 10.1158/0008-5472.CAN-08-1648

Figure 1. Targeted disruption of TGFBI in mice.

Figure 1

(A) Strategy for generating the targeted TGFBI allele. Exons 4–6 were replaced by neomycin-resistance cassette (neo) with introduction of one BamH1 restriction site at 3’ terminal. Targeting construct and wild type allele are shown. Successful targeting will yield a 4.2 kb BamH1-restricted fragment in the neo allele. (B) Germinal transmission of the targeted TGFBI allele was identified by Southern blot. (C) Identification of deletion of exons 4–6 in KO MEFs by RT-PCR using a pair of primers specific for the upstream and downstream regions of exons 4–6. W: wild type; T: truncated. (D) Western blot of conditioned medium prepared from MEFs with indicated genotypic backgrounds. Mouse TGFBI recombinant protein was used as positive control (P).