Skip to main content
. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Endocrinology. 2006 Dec 7;148(3):1359–1366. doi: 10.1210/en.2006-0750

Figure 4. TNFα stimulated de novo synthesis of ceramide.

Figure 4

(A) L6 cell pellets were analyzed via liquid chromatography-mass spectrometry to determine all the known cellular ceramide species. The predominant species detected in 6 different experiments were C16 and C24:1 ceramide. (B) The change from control for C16 and C24:1 ceramide following TNFα (T) pretreatment followed by insulin (I) or in the presence of myriocin (M) as determined by LC-MS. Shown is the mean of 2 representative experiments.