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. Author manuscript; available in PMC: 2009 Oct 21.
Published in final edited form as: Biochemistry. 2008 Sep 25;47(42):11097–11109. doi: 10.1021/bi801287m

Figure 4.

Figure 4

(a) Radius of gyration, Rg, as a function of pressure for 10 g/l L99A in 50 mM glycine 100 mM NaCl pH 3.0 buffer at room temperature. Rg was determined by a Guinier fit to the qRg < 1.3 region of scattering profiles taken at each pressure. The error bars are larger at high pressure because only one exposure was taken at each pressure. At lower pressure, the sample was less susceptible to radiation damage-induced aggregation and multiple exposures were taken at each pressure, which enabled averaging of images. A two-state thermodynamic fit is shown (solid line) to guide the eye. (b) The zero-angle scattering intensity of L99A (circle) and the WT* (diamond) at pH 3.0. WT* does not denature below 300 MPa.