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Denaturation volume changes predicted by side-chain volumes
| ΔV°predict (Å3) | δΔV (Å3) | |
|---|---|---|
| L99G/E108V | 206.2 ± 10.0 | 29.6 ± 26.9 |
| A98L | 73.1 ± 10.0 | 54.1 ± 12.1 |
| V149G | 187.1 ± 10.0 | -53.0 ± 12.4 |
The experimental denaturation volume change, ΔV°, of L99A at pH 3.0 20 mM NaCl was used as a reference. δΔV = ΔV°experiment - ΔV°predict. Volume changes of other mutants were predicted with van der Waals volumes of side-chains (52) of exchanged amino acids.
