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. 2009 Apr 9;4(4):e5159. doi: 10.1371/journal.pone.0005159

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) RT-PCR analysis demonstrating the presence of transcripts of M₃ muscarinic receptor (226 bp), M₅ muscarinic receptor (230 bp), ERα estrogen receptor (453 bp), ERβ estrogen receptor (204 bp) and GPR30 estrogen receptor (386 bp) in TM-BBB cells. (B) TM-BBB cells were stimulated with the estrogen receptor agonists, PPT (ERα), DPN (ERβ), or G1 (GPR30) for 60 min, and cell lysates were analyzed by western blotting with anti-pERK or anti-total ERK antibodies.