Fig. 6.

Histone modifications. (A) Effect of genistein and 5Aza-C on the histone modifications of the BTG3 promoter. ChIP assay was performed on cells after treatment with 50 μM/l genistein and 5 μM/l 5Aza-C. Untreated control (0), 5Aza-C (5A), 50 μM Genistein (50G). (B) Histone modification enrichment data calculated from the corresponding DNA fragments amplified by PCR at annealing temperature of 60°C for a total of 28 cycles; bars, error ± standard deviation. The amplified DNA was electrophoresed in a 2% agarose gel and visualized by staining with ethidium bromide. The ImageJ Software version 1.36b (http://rsb.info.nih.gov/ij/) was used for optical densitometry. Enrichment was calculated as the ratio between the net intensity of each bound sample normalized to its input sample and the vehicle control sample normalized to vehicle control input samples (Bound sample/Bound sample Input)/(Vehicle control sample/Vehicle control Input).