Figure 2. Expression analyses of cas genes.

Real time PCR was performed with total RNA isolated from S. macrospora wild type grown at 27°C in liquid BMM for three, five and seven days under ambient air conditions or 5% CO₂. Comparisons are given as logarithmic values of the ambient air/5% CO₂ ratios and are mean expression ratios from two independent biological replicates, each done in triplicate. For normalization, transcript levels of the SSUrRNA were calculated as described in Material and Methods. Asterisk indicate significance according to REST [70].