Figure 4. Expression of CaZF and salt tolerance of transgenic tobacco.

A, Northern blot showing expression of CaZF in T₁ transgenic tobacco lines transformed with pBI121 without (vector) or with CaZF (L21, L1, L17 and L46). Full length CaZF cDNA was used as probe. B, Vector control and T₁ transgenic progenies were grown on 0.5 MS (Murashige and Skoog) medium for 10 d. C, Effect of salt stress on tobacco seedlings from vector control and T₁ transgenic progenies (CaZFL21, CaZFL1, CaZFL17 and CaZFL46) was demonstrated by germinating seeds on 0.5 MS medium supplemented with 200 mM NaCl for 20 d. Representative figure of three independent experiments are shown. D, Chlorophyll content determined by leaf disc senescence assay for salinity tolerance of 30d-old vector control and transgenic tobacco lines overexpressing CaZF, after incubation in water, 150 mM and 300 mM NaCl solutions for 72 hr under continuous white light at 25±2°C. Results of three independent experiments are shown.