Table 2. Peptides enriched in tumor tissue after selection for tumor-homing AAV.
| Selection pathway | Peptide a | Charge pattern b | Frequency in selection round c | |||
| round 1 | round 2 | round 3 | round 4 | |||
| Pathway B | GGLSGVS | yyxxyyx | -/6 | -/7 | 1/22 | 7/41 |
| GGLSGDS | yyxxy-x | -/6 | -/7 | -/22 | 1/41 | |
| GSVSGSA | yxyxyxy | -/6 | -/7 | -/22 | 1/41 | |
| EYRDSSG | -y+-xxy | -/6 | -/7 | -/22 | 7/41 | |
| QMSGGVA | xyxyyyy | -/6 | -/7 | -/22 | 1/41 | |
| ESGLSQS | -xyyxxx | -/6 | 1/7 | 1/22 | 2/41 | |
| ESGIWVA | -xyyyyy | -/6 | -/7 | 1/22 | 2/41 | |
| EEPALRA | --yyy+y | -/6 | -/7 | -/22 | 4/41 | |
| APTLGSP | yyxyyxy | -/6 | 1/7 | -/22 | 13/41 | |
| Pathway B d | RGDLGLS | +y-yyyx | 5/16 | -/10 | ||
| DLGSARA | -yyxy+y | 2/16 | 10/10 | |||
| DGLGRLV | -yyy+yy | 6/16 | -/10 | |||
| DLRGLAS | -y+yyyx | 1/16 | -/10 | |||
| DRSPLSL | -+xyyxy | 1/16 | -/10 | |||
| Pathway C | AISGVGS | yyxyyyx | -/6 | 1/15 | 2/24 | 2/32 |
| DRSGVGS | -+xyyyx | -/6 | 1/15 | 4/24 | 2/32 | |
| SISGVGS | xyxyyyx | -/6 | -/15 | -/24 | 1/32 | |
| SEGRSGV | x-y+xyy | -/6 | -/15 | -/24 | 1/32 | |
| GEARSRA | y-y+x+y | -/6 | -/15 | -/24 | 1/32 | |
| GEARISA | y-y+yxy | -/6 | -/15 | 2/24 | 7/32 | |
| SGNSGAA | xyxxyyy | -/6 | 1/15 | 4/24 | 8/32 | |
| SSGSGGA | xxyxyyy | -/6 | -/15 | 2/24 | 2/32 | |
| ESGIWVA | -xyyyyy | -/6 | -/15 | -/24 | 2/32 | |
a
single letter code; only peptides occurring repetitively or sharing common sequence motifs are shown; shared amino acid patterns are highlighted in colored letters
b
charge pattern of amino acid side chains: +, positively charged; − negatively charged; x, uncharged polar; y, nonpolar.
c
observed frequency relative to overall number of sequenced clones
d
pathway B subsequent to 2 rounds of in vitro selection as in pathway A