Figure 2. Estrogen receptors form a complex with β-catenin and GSK3 in N2a-m cells.

ERα and ERβ were immunoprecipitated from N2a-m cells, and similar endogenous protein complexes were observed with both antibodies. Immunoprecipitation with an anti-APC antibody is shown as an internal control of a β-catenin interacting protein. Both GSK3 isoforms were detected in the immunocomplex recovered with ERα or ERβ antibodies, whereas GSK3β is the major isoform recovered with APC antibodies, used as a positive control. The immunoprecipitation also shows a fraction of β-catenin associated with both ER isoforms. IgG's represent the IP using irrelevant IgGs as a negative control.