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. 2009 Apr 10;4(4):e5153. doi: 10.1371/journal.pone.0005153

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Varea et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The scheme represents the structure of the 2.8 kB of construct containing the proximal region of endogenous engrailed 1 promoter bind to luciferase reporter. N2a-m cells were transfected with the pENP1-luciferase reporter plasmid (250, 200 and 750 ng) (represented as 0.25, 0.5, 0.75), which contains three LEF-1 sites (see upper panel). The Lower panel shows a comparison of the induction of TOPFlash (750 ng) and pENP-luc in this cell line. Although basal levels of luciferase activity are lower in the pENP1-luc reporter plasmid, estradiol induces this activity to 3-fold that of the control levels, as shown in the right panel. The graph in B shows the normalized luciferase activity from at least three independent experiments. The P value from the Student's t-test was ** (P≤0.01) when compared with control data.