Skip to main content
. 2009 Apr 10;4(4):e5153. doi: 10.1371/journal.pone.0005153

Figure 7. The LEF-1-DNA complex is detected in nuclear protein extracts and it is modulated by estradiol.

Figure 7

(A)-Identification of the specific band by competition with non-labeled wt oligonucleotides. Nuclear proteins were obtained from N2a-m cells treated for 30 minutes with estradiol, ICI or Wnt3a, enabling specific TCF-DNA complexes to be detected. In control nuclear extracts the presence of a pre-established TCF-DNA complex was detected, which augmented slightly in the presence of estradiol and that decreased upon ICI treatment. The arrow indicates the complex. (B)-Analysis of the identity of the TCF-DNA complex. Antibodies against TCF1, LEF-1, or irrelevant IgGs (C) were added to the nuclear protein extracts and modification of the DNA/protein complex was evaluated. In addition, antibodies against TCF3 or ERα were used in parallel experiments, without producing any modification of this pattern (Supplementary Figure S4). Only in the case of the anti-LEF-1 antibody was a more slowly migrating band observed. The arrow with an asterisk indicates the appearance of a higher molecular weight complex.