Table 1. Results of the Microarray analysis of gene transcription.
| UPREGULATED: | ||
| RefSeq_NM | est_A vs B | Gene_Name |
| NM_007990.1 | 2,739 | Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) |
| NM_133198.1 | 2,676 | liver glycogen phosphorylase |
| NM_130888.1 | 2,457 | nuclear RNA export factor 7 |
| NM_025703.2 | 2,439 | RIKEN cDNA 3930402F23 gene |
| NM_183271.1 | 2,181 | RIKEN cDNA 1700049M11 gene |
| NM_008877.2 | 2,039 | plasminogen |
| NM_007407.2 | 2,034 | adenylate cyclase activating polypeptide 1 receptor 1 |
| D87975.1 | 1,994 | serine (or cysteine) proteinase inhibitor, clade F, member 1 |
| NM_019703.2 | 1,890 | phosphofructokinase, platelet |
| NM_010480.4 | 1,847 | heat shock protein 1, alpha |
| NM_054095.1 | 1,846 | EF hand calcium binding protein 2 |
| NM_013779.1 | 1,701 | melanoma antigen, family L, 2 |
| NM_021389.3 | 1,653 | SH3-domain kinase binding protein 1 |
| NM_009529.1 | 1,509 | Xlr-related, meiosis regulated |
| NM_133362.1 | 1,491 | erythroid differentiation regulator 1 |
| NM_183294.1 | 1,487 | cyclin-dependent kinase-like 1 (CDC2-related kinase) |
| NM_007833.1 | 1,462 | decorin |
| BC053970.1 | 1,337 | NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1 |
| NM_008144.3 | 1,335 | Bernardinelli-Seip congenital lipodystrophy 2 homolog (human) |
| NM_033325.1 | 1,332 | lysyl oxidase-like 2 |
| NM_175027.2 | 1,321 | cDNA sequence BC022692 |
| NM_146034.1 | 1,316 | meningioma expressed antigen 6 (coiled-coil proline-rich) |
| NM_177752.2 | 1,313 | hypothetical protein 6820428D13 |
| NM_025437.2 | 1,298 | eukaryotic translation initiation factor 1A, Y-linked |
| NM_019768.2 | 1,291 | mortality factor 4 like 2 |
| NM_008590.1 | 1,251 | mesoderm specific transcript |
| NM_019647.3 | 1,247 | ribosomal protein L21 |
| NM_031174.2 | 1,246 | Down syndrome cell adhesion molecule |
| NM_009093.1 | 1,237 | ribosomal protein S29 |
| NM_008200.1 | 1,225 | histocompatibility 2, D region locus 4 |
| NM_008033.2 | 1,222 | farnesyltransferase, CAAX box, alpha |
| NM_026712.1 | 1,216 | RIKEN cDNA 0610030H11 gene |
| NM_183203.1 | 1,202 | hypothetical protein 9130430E04 |
| NM_145628.2 | 1,200 | ubiquitin specific protease 11 |
| NM_207204.1 | 1,188 | RIKEN cDNA 4930519N13 gene |
| NM_025695.2 | 1,178 | SMC6 structural maintenance of chromosomes 6-like 1 (yeast) |
| NM_008183.2 | 1,177 | glutathione S-transferase, mu 2 |
| NM_145526.1 | 1,173 | purinergic receptor P2X, ligand-gated ion channel, 3 |
| NM_012035.1 | 1,136 | transient receptor potential cation channel, subfamily C, member 7 |
| NM_010206.1 | 1,135 | fibroblast growth factor receptor 1 |
| NM_146035.1 | 1,122 | mannoside acetylglucosaminyltransferase 2 |
| NM_175930.2 | 1,118 | Rap guanine nucleotide exchange factor (GEF) 5 |
| NM_013819.1 | 1,111 | histocompatibility 2, M region locus 3 |
| NM_008666.1 | 1,094 | myelin transcription factor 1-like |
| NM_029823.1 | 1,092 | RIKEN cDNA 6530401D17 gene |
| NM_172758.1 | 1,090 | cDNA sequence BC031853 |
| AK040457.1 | 1,085 | interleukin 27 receptor, alpha |
| NM_194355.1 | 1,079 | spire homolog 1 (Drosophila) |
| NM_007881.4 | 1,072 | dentatorubral pallidoluysian atrophy |
| NM_025522.1 | 1,064 | dehydrogenase/reductase (SDR family) member 7 |
| NM_008549.1 | 1,063 | mannosidase 2, alpha 1 |
| NM_010545.2 | 1,058 | Ia-associated invariant chain |
| NM_008810.2 | 1,058 | pyruvate dehydrogenase E1 alpha 1 |
| NM_175007.1 | 1,044 | amphiphysin |
| NM_028270.2 | 1,029 | aldehyde dehydrogenase 1 family, member B1 |
| NM_008737.1 | 1,024 | neuropilin |
| NM_008666.1 | 1,023 | myelin transcription factor 1-like |
| NM_025932.1 | 1,016 | synapse associated protein 1 |
| NM_010195.1 | 1,006 | G protein-coupled receptor 49 |
This Table represent a selection of annotated genes upregulated (red) and downregulated (green) by estradiol, sensitive to LEF-1 mutant. The list contains only those “annotated genes” with a log2R higher or lower than 1. A represent the control cell line (N2a-m-mock transfected). B represents the cell line transfected with delta-56-LEF-1. In the table est, represents treatment with estradiol; and Wnt, treatment with Wnt3a.
We labeled two genes (plasminogen and LEF-1) regulated by estradiol or by Wnt3a , both were subsequently validated at protein level.