Figure 5.

Volatile-dependent superoxide generation and the participation in [Ca²⁺]_c transients in Arabidopsis leaves. (A) Volatiles-dependent superoxide generation in the leaves from AQ plants. Data represent means ± SEs (n = 3–7). (B) The effect of ROS scavengers, Tiron (40 mM) or ascorbic acid (AsA, 10 mM) on the (E)-2-hexenal-promoted [Ca²⁺]_c transient. Inhibition rates against the peak amplitudes of luminescence in control experiments were shown. Data represent means ± SEs (n = 4). (C) Superoxide production in cell free system. CLA-containing medium were exposed to (E)-2-hexenal. CLA luminescence of the medium (control), with Tiron or ascorbic acid. Data represent means ± SEs (n = 3). # and *p < 0.05, the two-tailed Student's t test. rcu; relative chemiluminescence units.