Fig. 2.
Schematic representation of the adult ex vivo preparation used to stain axotomized cutaneous afferents (a) and intracellularly stained cells double-labeled for CGRP. b–g: Confocal micrographs of labeled somata from previously axotomized myelinated afferents double-labeled for neurobiotin (green) and CGRP immunohistochemistry (red); arrows indicate position of labeled somata in each. b,c: Putative guard hair follicle afferent. d,e: Putative D-hair follicle afferent. e,f: Putative nociceptor with recurving, flame-shaped arbor. All lacked intraganglionic arborizations and were CGRP negative. Scale bar = 20 μm.