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. 2000 Jan 4;97(1):234–239. doi: 10.1073/pnas.97.1.234

Figure 3.

Figure 3

Identification of three FRQ phosphorylation sites. (A) Schematic diagram of SFRQ deletion constructs. The dashed box represents the entire SFRQ ORF (aa 100–989). The black bars below indicate the locations of the deleted regions in different sFRQ constructs. “4” denotes sFRQ4; 4A-D represent subsections of “4.” (B) FRQ phosphorylation profiles in various sFRQ deletion strains. Cultures were grown in LL for at least 24 hr before harvesting. * marks the strains in which SFRQ is less phosphorylated than the wild-type SFRQ. The various FRQ phosphorylation forms were separated by using a longer than normal electrophoresis time to emphasize the mobility differences caused by phosphorylation. (C) The sequence of the 20-aa region deleted in sFRQ4C. The three potential phosphorylation sites conserved among different fungal frq homologs are indicated by *. The point mutations introduced are described in parentheses. (D) SFRQ phosphorylation profiles of the mutants containing point mutations at the three potential sites. The arrow indicates the phosphorylation band missing in the three mutants.