Figure 5.

Two different mutations at S513 result in differential responses of FRQ degradation. (A) Western blot analysis showing that the S513I and S513D mutations lead to the loss of the top SFRQ phosphorylation band. The arrow indicates the missing band in the mutants. (B) Western blot analysis showing that the S513I mutation dramatically slows down the degradation of SFRQ. Cultures were grown in LL for a day before CHX was added at time 0. (C) Grouped data showing densitometric analysis of Western blots from three independent experiments as in B. Bars = SD.