Table 3. Sites of HNE Adduction on ApoA1 Following Treatment of HDL with HNE or Oxidation of Plasma with AIPHa.
| AIPH (μM) |
HNE (μM) |
|||||||
|---|---|---|---|---|---|---|---|---|
| sequence | 100 | 1000 | 5000 | 1 | 10 | 100 | 1000 | |
| VK*DLATVYVDVLK | K12 | 1/3b | ||||||
| DLATVYVDVLK*DSGR | K23 | 1/3 | ||||||
| DLEEVK*AK | K94 | 2/3 | 3/3 | |||||
| AK*VQPYLDDFQK | K96 | 1/3 | 3/3 | |||||
| K*WQEEMELYR | K107 | 3/3 | ||||||
| LH*ELQEK | H135 | 3/3 | 3/3 | |||||
| AH*VDALR | H155 | 1/3 | 2/3 | 3/3 | 3/3 | 3/3 | 3/3 | |
| TH*LAPYSDELR | H162 | 2/3 | 3/3 | 3/3 | 2/3 | 3/3 | 3/3 | 3/3 |
| LAEYH*AK | H193 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 | 3/3 |
| ATEH*LSTLSEK | H199 | 1/3 | 3/3 | 3/3 | 3/3 | |||
| AK*PALEDLR | K208 | 1/3 | 3/3 | 3/3 | ||||
| VSFLSALEEYTK*K | K238 | 1/3 | ||||||
a
Mass error (ppm) for measurements of the precursor ions of the observed peptide adducts were analyzed on a Thermo LTQ-Orbitrap instrument and were as follows: K12, 3.2 ppm; K23, 3.8 ppm; K94, 3.9 ppm; K96, 3.8 ppm; K107, 3.7; H135, 4.0 ppm; H155, 3.2 ppm; H162, 2.9 ppm; H193, 3.7; H199, 3.3; K208, 3.8 ppm; K238, 5.9.
b
Denotes that the adducts were detected in either 1 of 3, 2 of 3, or 3 of 3 independent experiments, respectively.