Figure 4.

Schematic representation of the tryptic digest experiment and interpretation. If the enzyme is unfolded on the aggregate, it should be more susceptible to proteolysis compared to enzyme in the absence of aggregates (A). If the enzyme is in its native state when bound to the aggregate, identical to unbound enzyme, it should be degraded at a comparable rate (B). Although some trypsin may be bound to the aggregate, trypsin is used in excess to ensure that there is free, active trypsin in solution: (+) indicates that aggregates are present; (−) indicates free enzyme.