Table 1.
| Native |
Met-loss |
|||
|---|---|---|---|---|
| Protein | Em (mV) | ΔEm | Em (mV) | ΔEm |
| HT WT | 211 ± 1 | n.a. | -110 ± 1 | n.a. |
| Q64N | 217 ± 1 | +6 | -103 ± 2 | +7 |
| Q64V | 189 ± 1 | -22 | -102 ± 2 | +8 |
| Q64Y | 177 ± 3 | -34 | -104 ± 2 | +6 |
| Unfolded WTa | n.d. | -- | -74 ± 4 | +36 |
| PA WT | 277 ± 1 | n.a. | -89 ± 2 | n.a |
| N64Q | 252 ± 1 | -25 | -83 ± 2 | +6 |
| N64V | 223 ± 1 | -55 | -85 ± 2 | +5 |
| Unfolded WTb | n.d. | -- | -91 ± 5 | |
| HH WT | 260 ± 5 | n.a. | -52 ± 1 | n.a. |
| Unfoldeda | n.d. | -- | -62 ± 1 | -10 |
Midpoint potentials of native and Met-loss form, collected at pH 6.0, 10°C. The listed value is the average of at least three trials and error is given as the standard deviation.
a
Midpoint potential (Em) was determined in pH 6.0 citrate + KPi, 30°C, plus 8M GdnHCl.
b
Midpoint potential (Em) was determined in pH 6.0 citrate/KPi, 10°C, 8M GdnHCl.