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. 2009 Mar 26;28(9):1197–1207. doi: 10.1038/emboj.2009.78

Figure 4.

Figure 4

CtBP1/BARS and PLD are required for EGF-stimulated macropinocytosis. (A) COS7 cells were transfected with control or CtBP1/BARS-siRNA and cultured for 48 h. Cells were serum-starved for 1 h and stimulated with 100 ng/ml EGF in the presence of tetramethylrhodamine-labelled dextran for 8 min, washed, fixed and analysed for macropinocytosis by confocal microscopy. (B) CtBP1/BARS-siRNA-transfected cells were administrated with either control vehicle or purified recombinant CtBP1/BARS (200 nM) with the BioPORTER reagent. Macropinocytosis assay was carried out as above in the presence or absence of 0.2% 1-butanol or 2-butanol. One representative experiment from at least three separate experiments is shown here. Bars, 10 μm. (C) Quantification of macropinocytosing cells treated as in (B). Data are means±s.e. from at least three independent experiments carried out in triplicate.