Figure 5.

Requirement of CtBP1/BARS for agonist-induced PLD activation. COS7 cells were transfected either with control or with CtBP1/BARS-siRNA and cultured for 30 h. Cells were then metabolically labelled with radioactive lysophosphatidylcholine for 18 h in the absence of serum. After stimulation of cells with 100 ng/ml EGF for 8 min or with various concentrations of fetal calf serum for 30 min in the presence of 0.3% 1-butanol, accumulation of PtdBut in the cells was measured and expressed as a percentage of total radioactivity. Data are the means±s.e. of three independent experiments carried out in triplicate. PtdBut, phosphatidylbutanol. ^*P<0.05 and ^**P<0.01 compared with control siRNA.