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. 2009 Mar 26;28(9):1197–1207. doi: 10.1038/emboj.2009.78

Figure 9.

Figure 9

Activation of PLD1 by CtBP1/BARS in a purified recombinant protein system. PLD1 (A) and PLD2 (B) activities were measured as a function of time in the absence or presence of 10 nM CtBP1/BARS using mixed phospholipid vesicles. PLD1 activity was reconstituted with various combinations of 1 nM CtBP1/BARS and 50 nM ARF1 in the absence or presence of 100 μM guanine nucleotides (C) or with various combinations of 1 nM CtBP1/BARS, 1 nM CtBP1/BARS(S147D), 4 nM ARF6, 300 nM RhoA and 100 nM PKCα (D). Enzymatic reactions proceeded for 20 min and PtdEtOH formation was measured. Data presented are means±s.e. of at least six independent experiments carried out in triplicate. *P<0.05 compared with no addition of activators. PtdEtOH, phosphatidylethanol.