Table 1.

Sequence of the primers used for the first PCR in the single-cell multiplex reverse transcription-PCR protocol

	First primer pair
	Sense PCR primers		Antisense PCR primers
Gene	Position	Sequence	Position	Sequence	PCR product
TrkB	916–935	ACTGTGAGAGGCAACCCCAA	1327–1346	ATCACCAGCAGGCAGAATCC	431
p75	151–170	GGAGCCAACCAGACCGTGTG	410–431	TCTGGGCACTCTTCACACACTG	280
BDNF	370–389	ATGTCTATGAGGGTTCGGCG	606–625	GCGAGTTCCAGTGCCTTTTG	256
VGlut2	316–335	CGCGGAGGCAAAGTTATCAA	805–828	TCTGTAATGGTAGGATGCTTTGC	513
NK1R	34–53	TTCCCCAACACCTCCACCAA	455–474	AGCCAGGACCCAGATGACAA	441
Mu-R	75–94	ACCTGGCTCCTGGCTCAACT	290–310	TGGTGGCAGTCTTCATTTTGG	236
HCN1	1642–1663	TGTCGTCTTTACTCCCTTTCGG	1956–1975	CGGTGTAGACTGGCGGAGAT	334
HCN2	1698–1718	CAAGGGCAACAAGGAGATGAA	2056–2073	TGGCGGTGGTGGGAAGAG	376
HCN3	1530–1549	TTGTCGCCTCTACTCGCTCA	1902–1921	GGAGGGTGGCTGGAGAATCA	392
HCN4	24–43	CCGCTATCAAAGTGGAGGGA	265–284	TCAGCAACAGCATCGTCAGG	261
Intron	8–27	CTGTCCCCCTTACGAATCCC	228–247	CCAGCACCAGGGATAGAGCC	220

The primers are designed from 5′ to 3′. Position 1 corresponds to the ATG codon.