Table 2.

Sequence of the primers used for the second PCR in the single-cell multiplex reverse transcription-PCR protocol

	Second primer pair
	Sense PCR primers		Antisense PCR primers
Gene	Position	Sequence	Position	Sequence	PCR product
TrkB	941–962	CGCTTCAGTGGTTCTACAATGG	1265–1284	CTCCCGATTGCTTTGGTCAG	344
p75	161–180	AGACCGTGTGTGAACCCTGC	364–381	GAGTCCTGAGCCCACCCC	221
BDNF	445–464	TGGGTCACAGCGGCAGATAA	575–595	TGCAGCCTTCCTTGGTGTAAC	151
VGlut2	362–379	CCGAGACCGTGGGGATGA	773–796	CATAAGACACCAGAAGCCAGAACA	435
NK1R	123–142	CATCGTGGTGACTTCCGTGG	397–416	TGAAGAGGGTGGATGATGGC	294
Mu-R	196–216	GTCACAGCCATCACCATCATG	287–310	TGGTGGCAGTCTTCATTTTGGTAT	115
HCN1	1711–1734	GCCTTTGAGACAGTTGCTATTGAC	1872–1895	TGAGGATAGTTGATTGGAGGGATA	185
HCN2	1830–1849	TTTCAACGAGGTGCTGGAGG	1968–1987	TGGCATTCTCCTGGTTGTTG	158
HCN3	1564–1583	AATGCGGTGCTTGAGGAGTT	1885–1902	AGGGGAGAGGGGCAGAGG	339
HCN4	24–43	CCGCTATCAAAGTGGAGGGA	254–275	GCATCGTCAGGTCCCAGTAAAA	252
Intron	16–35	CTTACGAATCCCCCAGCCTT	178–197	TTGAAAGCCAGGGAGGAACT	182

The primers are designed from 5′ to 3′. Position 1 corresponds to the ATG codon.