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. 2009 Mar 17;10:113. doi: 10.1186/1471-2164-10-113

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Copyright © 2009 Slevin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A, (i) Area with discreet region of CD105-positive (red; circle) and CD105-negative (blue: circle) vessels captured with the laser. (ii) RNA extraction from multiple combined laser cut sections showing good RIN and concentration. B, (i-ii) Shows non-significant differences in ΔCts between pre-amplified genes indicating no bias in gene amplification. (iii) Significant increases in gene expression of CD105 and Flt-1 in the positively selected samples indicated the sampling/IHC was accurate. (iv) Significant correlation between CD105 and Flt-1 expression in all the tested samples indicated reliability of the markers to discriminate between active and inactive vessels.