Skip to main content
. Author manuscript; available in PMC: 2009 Apr 2.
Published in final edited form as: Nat Protoc. 2008;3(9):1402–1413. doi: 10.1038/nprot.2008.120

Figure 5. An example of cell-surface flow cytometry.

Figure 5

The intensity of the phycoerythrin (PE) signal among the 10,000 GFP-positive cells is measured and plotted. Rho-Olfr62 is cotransfected with and without RTP1S. Robust Olfr62 expression is seen as right-shifted plots when RTP1S is cotransfected and cells are stained with anti-Rhodopsin antibody (red line). Lack of cell-surface expression is seen when no RTP1S is cotransfected (compare blue line and negative control black line).