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. 2009 Apr 3;15:662–669.

Figure 4.

Figure 4

BDNF-positive cells of sections of the SC. A: Representative microphotographs of the superior colliculus (SC; the contralateral side and the ipsilateral side) are shown for the control group (untreated) 3, 7, 30, 90, and 180 days after N-methyl-D-aspartate (NMDA) injection. The scale bar represents 30 µm. B: Representative photographs show brain-derived neurotrophic factor (BDNF) and neuronal nuclear specific protein (NeuN) immunostaining, and BDNF/NeuN double-immunostaining of the contralateral SC at 30 days after intravitreal NMDA injection in mice. Some BDNF-expressing cells were colocalized with NeuN-positive neuronal cells, as indicated by the yellow color. The scale bar represents 20 µm. C: The average number of BDNF immunopositive puncta was counted in the SC. Each value represents the mean±SEM (n=3). The asterisk indicates p<0.05, while the double asterisk represents p<0.01 versus control (untreated mice; Dunnett’s test).