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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Cancer Res. 2009 Mar 10;69(7):2887–2895. doi: 10.1158/0008-5472.CAN-08-3343

Figure 4. Mesenchymal cell lines have elevated expression of drug pumps, DNA repair enzymes and are resistant to environmental insults.

Figure 4

Panel A (left) shows western blot analysis of BCRP and PGP drug pumps in all cell lines. β actin was used as a loading control. Western blots are representative of three experiments for each protein. Panel A (middle) shows inhibition of growth (i.e. compared to control treated cells) of the tumor cells lines after in vitro treatment with mitoxantrone. Panel A (right) shows the apoptotic response to etoposide as assessed using flow cytometry. Each bar is expressed as the % apoptotic cells following treatment. A representative experiment is shown for MTT and apoptotic assays, both performed twice. Panel B (left) depicts the expression levels of MGMT in the tumor cell lines by real time PCR. Results are expressed as fold expression over E and represent one experiment. Panel B (right) shows the results of a clonogenic assay of the tumor cells following exposure to escalating doses of BCNU. Each data point is the mean (s.e.m.) of 3 replicates. Panel C (left) shows the results of real-time PCR analysis of the double stranded DNA repair genes. Results are expressed as fold expression over E. Panel C (right) shows the results of clonogenic assay after escalating doses of gamma irradiation. Each data point is the mean (± s.e.m.) of 3 replicate plates.