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. Author manuscript; available in PMC: 2009 Apr 3.
Published in final edited form as: J Invest Dermatol. 2000 Aug;115(2):234–244. doi: 10.1046/j.1523-1747.2000.00034.x

Figure 5. Delayed in vitro wound closure in keratinocytes from CXCR2−/− mice.

Figure 5

In vitro wounding of confluent cultures of CXCR2+/−, and −/− keratinocytes. Keratinocytes from the three genotypes (+/+, +/−, and −/−) were isolated and cultured in 24 well tissue culture plates as described in Materials and Methods. Wounds (400–500 μm) on monolayered keratinocytes were monitored over a period of 24 h and the area of the wound defect determined using Bioquant software. Keratinocytes from wild-type mice exhibited a wound closure which was not significantly different from keratinocytes of heterozygous mice. Part (a) shows the percentage of wound closure of keratinocytes from +/− mice compared with −/− mice during a 24 h period. Values represent mean ± SEM obtained from cultures derived from three different mice and five independent wounds in each well. Part (b) depicts representative in vitro photographs of wounded keratinocyte cultures from +/+ (A–C) and −/− (D–F) mice during wound closure. Scale bar. 200 μm.