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. 2009 Apr 13;4(4):e5121. doi: 10.1371/journal.pone.0005121

Figure 1. Shh expression commences late in S. canicula (Sc) fin development, concomitant with Hoxd13 expression.

Figure 1

(A–G) Pectoral fin buds. Anterior is to the left. (A) ScShh expression at stages 27, 29 and early stage 32. Transcripts were present in the posterior region (arrowheads) at stage 29 but absent at stages 27 and early stage 32. (B, C) Expression of ScHoxa11 (B) and ScHoxa13 (C). ScHoxa11 transcripts were first detected in the posterior region and in the muscle buds. By early stage 32, transcripts were restricted to the posterior-distal region. ScHoxa13 transcripts were restricted to the distal part of the fin buds throughout fin development. Arrowheads indicate limits of ScHoxa expression. (D–G) Expression of ScHoxd10 (D), ScHoxd11 (E), ScHoxd12 (F) and ScHoxd13 (G). The ScHoxd genes were expressed collinearly at early stages. ScHoxd10–d12 transcripts were apparent at stage 27, whereas ScHoxd13 transcripts were first observed in the posterior mesenchyme at stage 29. Arrowheads indicate the anterior limits of ScHoxd expression. (H) Quantitative PCR analysis to determine the expression levels of ScHoxd10–13 in the pectoral fins of stage 26, 27 and 29 dogfish embryos. Relative expression was normalized against ScGAPDH transcripts. Note that levels of ScHoxd10–13 transcript expression increased at stage 29. Expression of ScHoxd10–d13 in stage 26 pectoral fins, or expression of ScHoxd13 in stage 27 pectoral fins, was not detectable. (I) Schematic representation of temporal Hoxd expression and Shh expression during pectoral fin development in S. canicula. Shh was expressed concomitantly with Hoxd13.