Localization of collagen, mineral deposits, DMP1, DPP, and DSP in a postnatal 5 day mouse first molar. Deposition of collagen and mineralized matrix are observed (A,A′,B,B′). Localization of DMP1, DPP, and DSP were predominantly observed at the mineralization front (C′–E′, arrow). All three matrix proteins were also localized in the nucleus of pulp cells. The insert in E depicts the localization of DSP throughout the cytoplasm in the odontoblasts. A′–E′ are higher magnification from the corresponding boxed portion from A–E. P, pulp; O, odontoblasts; Am, ameloblasts. Bars: A–E = 20 μm; A′–E′ = 10 μm.