Table 2.
Quantitative analysis [mean ± SD (n)] of MTT assay (optical density, 570–650 nm), ALP activity (μmol thymolphthalein/hr/mg protein), total protein content (μg/ml), and proportion of bone-like nodule formation (%) of control, GFs + proteins–, GFs + proteins/10–, GFs + proteins/100–, and protein-treated rat calvarial osteogenic cell cultures grown on titanium discs
| MTT assay
|
ALP activity
|
Total protein content
|
Bone-like nodule formation
|
|||
|---|---|---|---|---|---|---|
| Groups | Day 7 | Day 7 | Day 10 | Day 7 | Day 10 | Day 14 |
| Control | 0.12 ± 0.01 (4) | 6.2 ± 2.6 (5) | 24.9 ± 5.4 (5) | 58.0 ± 3.2 (5) | 92.6 ± 3.4 (5) | 56.6 ± 10.1 (4) |
| GFs + proteins | 0.20 ± 0.02 (4) | 2.2 ± 1.4 (4) | 2.0 ± 0.6 (5) | 81.3 ± 7.4 (4) | 99.0 ± 10.1 (5) | 0.9 ± 0.3 (4) |
| GFs + proteins/10 | 0.13 ± 0.01 (4) | 1.0 ± 0.4 (5) | 5.0 ± 1.9 (5) | 67.5 ± 4.9 (5) | 96.0 ± 9.1 (5) | 0.7 ± 0.7 (4) |
| GFs + proteins/100 | 0.10 ± 0.01 (4) | 0.4 ± 0.9 (5) | 3.4 ± 2.0 (5) | 46.5 ± 3.1 (5) | 73.9 ± 5.8 (5) | 26.4 ± 1.4 (4) |
| Proteins | 0.12 ± 0.02 (4) | 1.2 ± 0.9 (4) | 8.6 ± 2.1 (4) | 53.9 ± 3.5 (4) | 87.0 ± 11.4 (4) | 46.8 ± 0.7 (4) |
| Kruskal-Wallis test | Sa | Sa | Sa | Sa | Sa | Sa |
a
Significant (p<0.01).
MTT, mitochondrial tetrazolium test; ALP, alkaline phosphatase; GF, growth factor.