Table 3.
Comparative analysis (Fischer post-test) for MTT, ALP activity, total protein content, and bone-like nodule formation of control, GFs + proteins–, GFs + proteins/10–, GFs + proteins/100–, and protein-treated rat calvarial osteogenic cell cultures grown on titanium discs
| MTT assay
|
ALP activity
|
Total protein content
|
Bone-like nodule formation
|
|||
|---|---|---|---|---|---|---|
| Comparisons (Fischer post-test) | Day 7 | Day 7 | Day 10 | Day 7 | Day 10 | Day 14 |
| Control × GFs + proteins | 1% | 5% | 0.1% | 0.1% | NSa | 0.1% |
| Control × GFs + proteins/10 | NSa | 0.1% | 0.1% | 0.1% | NSa | 0.1% |
| Control × GFs + proteins/100 | NSa | 0.1% | 0.1% | 0.1% | 1% | 0.1% |
| Control × proteins | NSa | 1% | 5% | 5% | NSa | NSa |
| GFs + proteins × GFs + proteins/10 | 5% | NSa | 0.1% | 1% | NSa | NSa |
| GFs + proteins × GFs + proteins/100 | 0.1% | 1% | 5% | 0.1% | 0.1% | 1% |
| GFs + proteins × proteins | 1% | NSa | 0.1% | 0.1% | 5% | 0.1% |
| GFs + proteins/10 × GFs + proteins/100 | 1% | NSa | NSa | 0.1% | 0.1% | 0.1% |
| GFs + proteins/10 × proteins | NSa | NSa | 5% | 0.1% | NSa | 0.1% |
| GFs + proteins/100 × proteins | 5% | NSa | 0.1% | 1% | NSa | 1% |
a
Non-significant (>0.05).
MTT, mitochondrial tetrazolium test; ALP, alkaline phosphatase; GF, growth factor.