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. 2009 Apr 13;4(4):e5249. doi: 10.1371/journal.pone.0005249

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Deng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A DNA bead-assembled bipolar spindle (arrow) in MII eggs (MII, maternal chromosome-induced spindle). (B) DNA bead-spindle underwent metaphase-anaphase transition and induced polar body extrusion after egg activation. Note that the DNA beads were moved to one spindle pole and discarded in the extruded polar body (arrow). (C) DNA bead and meiotic chromosome poleward movements preceded the polar body extrusion. The arrow indicates the DNA beads and arrowheads show the segregated meiotic chromosomes. Note that the polar body was not yet extruded in this egg. The arrowheads show the segregated meiotic chromosomes. (D) Survivin (in red) localization at the spindle midzone (arrow) of the DNA bead-spindle (the same egg shown in panel B). (E) DNA bead poleward movement on a spindle formed far away from the cortex. The arrow indicates the DNA beads and the arrowheads indicate the two sets of segregated meiotic chromosomes. (F) Poleward movement of injected sperm chromatin to one spindle pole (arrow). The arrowheads show the segregated meiotic chromosomes. (G) Co-injected DNA beads (arrowhead) and sperm chromatin (arrow) sharing the same metaphase spindle. (H) After anaphase onset, DNA beads (arrowhead) and sperm chromatin (arrow) segregated from each other and moved to opposite poles in 7/20 oocytes. The unmarked spindle is maternal. (I) Both DNA beads (arrowhead) and sperm chromatin (arrow) moved to the same spindle pole in 13/20 oocytes. The unmarked spindle is maternal.