Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr 13;4(4):e5185. doi: 10.1371/journal.pone.0005185

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Aranko et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(a) SDS-PAGE analysis of in vivo protein ligations by the newly engineered split SspDnaE inteins after purification with Ni-NTA. The combinations of SspDnaE-Int_N and SspDnaE-Int_C are indicated on the top of the lanes. (b) In vivo protein ligation by NpuDnaE intein with the newly engineered split site (NpuDnaE-Int_N123/C15). Lane 1, before induction; lane 2, 1.5 hours after induction only with arabinose; lane 3, 1.5 hours after additional induction with IPTG; lane 4, 3 hours after induction with IPTG and arabinose; lane 5, elution from Ni-NTA column.