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. 2009 Apr 13;4(4):e5185. doi: 10.1371/journal.pone.0005185

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Aranko et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Time course of the protein ligation of nSH3 and GB1 by naturally occurring split NpuDnaE intein in the presence of 50 mM DTT. Lane 1, 0 min after the mixing; lane 2, 3 min; lane 3, 10 min; lane 4, 30 min; lane 5, 1 hour; lane 6, 3 hours; lane 7, 22 hours. (b) Kinetic analysis of the protein ligation from the SDS-PAGE. (c) SDS-PAGE analysis of the ligation reaction after overnight incubation in the presence of different reducing agents.